Marmara Pharmaceutical Journal 2017 , Vol 21 , Issue 4
Characterization of Sideritis trojana Bornm. essential oil and its antimicrobial activity
Hasan KIRMIZIBEKMEZ1,Nursenem KARACA2,Betül DEMİRCİ2,Fatih DEMİRCİ3
1Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR- 34755, Kayışdağı, İstanbul, Turkey
2Department of Pharmacognosy, Graduate School of Health Sciences, Anadolu University, TR-26470 Eskişehir, Turkey
3Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, TR-26470 Eskişehir, Turkey
4Faculty of Health Sciences, Anadolu University, TR-26470 Eskişehir, Turkey
DOI : 10.12991/mpj.2017.14 The components of the essential oil obtained from the dried aerial parts of Sideritis trojana Bornm. by hydrodistillation was analyzed both by GC-FID and GC-MS, simultaneously. Overall 57 compounds were identified representing 83.8% of the oil. The major components of the oil were identified as valeranone (11.3%), α-bisabolol (10.9%) and β-caryophyllene (8.8%), respectively. The composition of the oil showed quantitative chemical variation from previously studied material in terms of its major components. Moreover, the essential oil was evaluated for its in vitro antibacterial and anticandidal activities using a broth microdilution method. A selected panel of standard strains of Gram (+) and Gram (-) human pathogens as well as Candida albicans were used in the assay. As a preliminary result, it was observed that the oil displayed relatively moderate antibacterial activity against Helicobacter pylori with MIC value of 250 μg/mL when compared to standard antimicrobials. As a conclusion, it is worthwhile to evaluate the plant material against a broader spectrum of activities. Keywords : Sideritis trojana; Lamiaceae; essential oil; GC-FID and GC-MS; antimicrobial; Helicobacter pylori