Marmara Pharmaceutical Journal 2017 , Vol 21 , Issue 4
Anti-adenovirus activity, antioxidant potential, and phenolic content of dried flower buds of Syzygium aromaticum extract in HEp2 cell line
Mohammad-Taghi MORADI1,Ali KARIMI2,Somayeh ALIDADI3,Leila HASHEMI4
1Student research committee, Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
2Medical Plants Research Center; Shahrekord University of Medical Science, Shahrekord, Iran
3Clinical Biochemistry Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
4Cellular and Molecular Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
DOI : 10.12991/mpj.2017.4 The aim of this study is to evaluate in vitro anti-adenovirus property, antioxidant potential, and total phenolic content of dried flowers buds of Syzygium aromaticum crude extract. The crud extract was prepared and its anti-adenovirus activity was investigated on HEp2 cell line using MTT (3-[4,5-dimethylthiazol–2-yl]-2,5-diphenyltetrazolium bromide) assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu method and aluminum chloride colorimetric method was used to determine antioxidant activity, total phenol content, flavonoids and flavonols content of the extract, respectively. Based on results, the 50% cytotoxicity concentration (CC50) and the 50% inhibitory concentration (IC50) of the extract were 97.66±11.4 and 4.73±1.6 μg/ml, respectively, with the selectivity index (SI) of 20.64. The crud extract inhibited adenovirus replication in post-adsorption step (p<0.05). The extract showed remarkable scavenging activity with IC50 values of 10.05±1.93 μg/ml. Total phenolic, flavonoid and flavonol content of the crude extract was 255.8±3.95 mg GAE/g, 63.9±2.35 mg RUT/g and 62±2.35 mg RUT/g, respectively. The results of the present study indicated that S. aromaticum crude extract exhibited anti-adenovirus activity with inhibitory effect on adenovirus replication could be considered as a potential anti-adenovirus agents. Keywords : Antiviral; clove; Syzygium aromaticum; Adenovirus; antioxidant activity, Phenolic content